Category No: 94-11 AND
96-11
Syllabus
1. Anatomy, Physiology &
Biochemistry 1. Anatomy, Physiology & Biochemistry
Anatomy
Anatomical and histological
aspects of skeletal system, muscular system, joints, Thorax, Respiratory
system, Heart, Vascular System, Lymphatic System, Digestive System, Urinary
System, Respiratory System, Nervous System, Endocrine System, Skin and Tissues.
Physiology
Blood-composition and
functions, respiratory system, Digestive system, cardiovascular system, renal
system, endocrinology, reproductive system, muscle and nerve, nervous system,
skin and temperature regulation.
Biochemistry
Units of measurements,
laboratory glasswares, cleaning of glasswares, laboratory safety, laboratory
hazards, first aid, expressing concentrations of solutions, grades of
chemicals, purification of water, buffer systems, balance, centrifuge, calibration
of glasswares, primary and secondary standards, Collection and preservation of
biological specimens, colorimetry, spectrophotometry, flourimetry, nephalometry,
flame photometry, radioisotopes. Physical chemistry - osmosis, diffusion,
dialysis, Donnan-membrane equilibrium, viscosity, colloids, emulsions.Basic
knowledge about the metabolism of carbohydrate, protein, lipids, nucleic acid,
vitamins, minerals, haemoglobin, prophyrins, enzymes, acid-base balance,
nutrition, hormones. Function tests - liver, kidney, gastro intestinal, pancreas,
gonadal, feto-placental, thyroid function tests. Lipid profile , analysis of calculi,
electrophoresis, chromatographic techniques.CSF analysis ,urine qualitative and
quantitative analysis, Bence Jones Protein urine metabolite -VMA & 5- HIAA
and gastric juice analysis. Quality control programme and automation in a clinical
biochemistry laboratory.Estimation of various parameters present in the blood (
glucose, urea, cholesterol, uric acid, creatinine ,phosphorous, calcium,
electrolytes, Bilirubin ) samples and their normal values and interpretations,
GCT, GTT, Glycosylated haemoglobin. Clinical enzymology - ALP, ACP, Amylase,
AST, ALT, LDH, CK (activity determination, normal values, Interpretations etc).
2. Microbiology
General
Microbiology General Microbiology
History of microbiology,
laboratory safety. Microscopy - compound, dark ground, fluorescent and electron
microscope. Sterilization and disinfection - different agents and technique
used. Staining techniques - Gram's, Acid fast & other special staining
techniques. Morphological classification of bacteria, culture media,
composition & preparation of media, culture technique, biochemical tests
used for identifying bacteria, a study of medically important bacteria,
processing clinical specimen for diagnosing infections, serological tests for
diagnosis of infections, antibiotic susceptibility tests.
Parasitology
Medically important
protozoan and helminthic parasites and important protozoa, nematodes, cestodes
and trematodes their morphology, life cycle and laboratory diagnosis.Different
techniques used for the laboratory diagnosis of intestinal protozoan
infections, Trichomoniasis, malaria, leishmaniasis, trypanosomiasis, filariasis
, intestinal helminthic infections etc.
Mycology
A brief study of common
medically important fungi & their infections. Common contaminant fungi.
Morphological study - KOH preparation, Lacto phenol cotton blue, slide culture,
culture media - composition and preparation of Sabouraud's dextrose agar. Virology
- Structure of viruses, DNA & RNA viruses, cultivation of viruses, common
viral infections and laboratory diagnosis of infections.Immunology - Antigen,
Antibody , Antigen-Antibody reactions - agglutination, precipitation, ELISA,IF
and common serological techniques for diagnosing microbial infections. 3.
Pathology 3. Pathology
Haematology - Composition of blood,
development of blood cells. Normal and abnormal blood cell morphology,
functions and identification. Haemoglobin -function, normal and abnormal
haemoglobins and estimation. Anaemia, Leukemia,blood coagulation factors and
mechanism of coagulation, disorders of coagulation, anticoagulants, collection
of specimens, RBC, WBC, platelets and absolute eosinophil count, DC, ESR, PCV,
osmotic fragility, Romanowsky stain ,supra vital staining ,reticulocyte count,
red cell indices ( MCV, MCH, MCHC, CI). Bone marrow smear and staining.
Peroxidase, PAS staining and Perl's staining and their significance. Blood
parasites identification, LE cell preparation and identification. Blood coagulation
studies and disorders of coagulation ( BT, CT, PT, PTT & TT ).
Automatic blood
cell counter.
Clinical Pathology - Urine -
normal and abnormal constituents, physical, chemical and microscopic
examination, stool examination for parasites, sputum examination,semen
analysis, pregnancy test, CSF examination, examination of other body fluids.
Histotechnology -
Introduction to histology. Reception of specimen, fixation,decalcification,
processing of specimens (dehydration, clearing, infiltration and embedding).
Microtomes and cutting of sections, staining and mounting (haemotoxylin &
Eosin staining, PAS, Reticulin, VanGieson, Verhoeffs, Masson trichrome).
Processing of frozen sections, Histokinet, Cryostat.
Blood banking - ABO and other blood group
system Rh system. Antigen antibody
reactions in immune
haematology. Anticoagulants used in blood bank. Preparation of grouping sera.
Grouping and Rh typing of blood, screening of donors, collection, preservation
and storage of blood. Compatibility test in blood transfusion, serological
tests on donor blood. Transfusion reactions, transmission of diseases by blood
transfusion. Component separation.
Cytology - Important applications of
cytology, collection of various specimens and
preparation of smear, cell
blocks, cytospin, cytological fixatives Papanicoloau's staining, Shorr's
staining, identification of normal and abnormal smear, FNAC. Cytogenetics -
Buccal smear preparation, staining and Barr body identification. Karyotyping -
chromosome identification, abnormal chromosomes and chromosome defects.
NOTE: - It may be noted that apart from
the main topics listed above, questions from other topics prescribed for the
educational qualification of the post may
also appear in the question paper. There is no undertaking that all the topics above may be covered in the question
paper.
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